The 31st SMIIC MC Training within the SMIIC MC Work Plan 2021 – 2023 V2 was the Training on Nucleic Acid Based Measurement Methods for Determination of Pork Products in Food, Pharmaceuticals and Cosmetics that took place on the 19 September 2022 virtually, technically hosted by SMIIC General Secretariat. 17 experts participated in the training.
The training was hosted by NMI: Tübitak UME – Turkey
General Description: The training covers general and practical aspects of DNA based halal authenticity testing.
Objective, expected outcome: To provide knowledge and to develop skill of participants in Nucleic Acid based measurement methods for determination of pork products in food, pharmaceuticals and cosmetics.
Who should attend: The training would be attended by staff working in biometrology laboratories at the level of National Metrology Institutes or secondary level calibration laboratories.
References:
International vocabulary of metrology - Basic and general concepts and associated terms (VIM) Guide to the expression of uncertainty in measurement (GUM) ISO/IEC 17025:2016 Testing and Calibration Laboratories ISO 21570:2005 - Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods
Duration of training: 1 hours (19 September 2022)
Lecturer:
Burhanettin YALÇINKAYA PhD (burhanettin.yalcinkaya@tubitak.gov.tr)
Time Schedule: 10:30 – 16:00
Subject/Activities:
- General information about Nucleic Acid based measurements and application fields
- Separation of Nucleic Acids from matrix and quantitation
- Measurement principles of Nucleic Acids
- Primer and probe design for PCR
- Principles of Real Time PCR quantification
- Principles of Digital PCR quantification
- Method validation and uncertainty calculations
